Research

Biosynthesis of the plant cell wall, glycosyltransferases, abiotic stress.
Biosynthesis and regulation of the plant cell walls

Polysaccharides constitute the major part of plant cell walls. There are many different types of polysaccharides and a large number of enzymes are needed for their biosynthesis. The rational development of biofuel crops with improved properties will require a much better understanding of cell wall biosynthesis and the role of cell wall polymers for plant growth and development. In the Cell Wall Biosynthesis Group we aim to:

- Identify the glycosyltransferases and accessory enzymes required for biosynthesis of matrix polysaccharides and glycoproteins in the cell wall.

- Develop efficient methods for biochemical analysis of cell wall biosynthetic enzymes

- Use the knowledge about cell wall biosynthesis to make targeted modifications of cell wall composition in transgenic plants and determine the effects on plant growth and biofuel yield.

- Participate in forward genetic screens for plants with improved properties for biofuel production.

The polysaccharides in plant cell walls are often grouped into cellulose, hemicellulose and pectin. The major hemicellulose in grasses and in secondary walls is xylan. Arabinoxylans are synthesized from UDP-D-xylose and UDP-L-arabinose by the action of glycosyltransferases in the Golgi vesicles. The sugar residues are added onto existing acceptor molecules, which may be oligo- or polysaccharides. Xylans can be acetylated and cereal arabinoxylans also contain esterlinked ferulic and p-coumaric acid the biosynthesis of which is not understood. The acetyl and feruloyl esters are a major challenge for biofuel production. In order to characterize the individual glycosyltransferases and other biosynthetic enzymes it is essential to develop assays that are dependent on heterologously expressed enzymes and exogenous acceptor molecules.

Abiotic stress

Plants are exposed to many different kinds of abiotic stress. Since biofuel crops are likely to be grown on marginal lands, it is essential that they will be able to grow well under stressful conditions.

We are interested in tolerance to drought stress and to temperature stress. Heat and cold stress damage the photosynthetic apparatus, and we want to investigate the mechanism that plants use to recovery after such stress. Recovery is very slow and hence even a stress exposure of one hour can affect the ability of the plant to grow for several weeks. We are also investigating if some of the mechanisms that 'primitive' plants and microorganisms use to tolerate drought stress can be transferred to higher plants.

Recent Publications

2013

Geshi N, Johansen J, Dilokpimol A, Rolland A, Belcram K, Kotake T, Tsumuraya Y, Kaneko S, Tryfona T, Dupree P, Verger S, Scheller HV, Höfte H, Mouille G (2013) A galactosyltransferase acting on arabinogalactan protein glycans is essential for embryo development in Arabidopsis. Plant J, in press.

Chiniquy D, Varanasi P, Oh T, Harholt J, Katnelson J, Singh S, Auer M, Simmons B, Adams PD, Scheller HV, Ronald PC (2013) Three novel rice genes closely related to the Arabidopsis IRX9, IRX9L, and IRX14 genes and their roles in xylan biosynthesis. Frontiers Plant Biotechnol 4: 83. Published online 2013 April 10. doi:  10.3389/fpls.2013.00083

Gille S, Sharma V, Baidoo EEK, Keasling JD, Scheller HV, Pauly M (2013). Arabinosylation of a Yariv precipitable cell wall polymer impacts plant growth as exemplified by the arabidopsis glycosyltransferase mutant ray1. Mol Plant (2013) doi: 10.1093/mp/sst029 First published online: February 9, 2013

Bartley LE, Peck ML, Kim S-R, Ebert B, Manisseri C, Chiniquy D, Sykes R, Gao L, Rautengarten C, Vega-Sanchez ME, Benke PI, Canlas PE, Cao P, Brewer S, Lin F, Smith WL, Zhang X, Keasling JD, Jentoft RE, Foster SB, Zhou J, Ziebell A, An G, Scheller HV, Ronald PC (2013) Overexpression of a BAHD Acyltransferase, OsAt10, alters rice cell wall hydroxycinnamic acid content and saccharification. Plant Physiol 161(4): 1615-1633. Published online 2013 February 7. doi:  10.1104/pp.112.208694

Oikawa A, Lund CH, Sakuragi Y, Scheller HV (2013) Golgi-localized enzyme complexes for plant cell wall biosynthesis. Trends Plant Sci 18(1): 49-58. doi: 10.1016/j.tplants.2012.07.002. Epub 2012 Aug 25.

Vega-Sánchez ME, Verhertbruggen Y, Scheller HV, Ronald PC (2013) Abundance of mixed linkage glucan in mature tissues and secondary cell walls of grasses. J Plant Signaling Behavior 8: e23143. [Epub ahead of print]

Chen X, Vega-Sánchez ME, Bartley L, Verhertbruggen Y, Chiniquy D, Canlas P, Fagerström A, Prak L, Christensen U, Oikawa A, Chen M, Zuo S, Auer M, Willats W G.T., Harholt J, Scheller HV, and Ronald PC. (2013) Inactivation of OsIRX10 leads to decreased xylan content in rice stem cell walls and improved biomass saccharification. Mol Plant 6(2): 570-573. doi: 10.1093/mp/sss135. Epub 2012 Nov 23.

2012

Vega-Sánchez ME, Verhertbruggen Y, Scheller HV, Ronald PC. Abundance of mixed linkage glucan in mature tissues and secondary cell walls of grasses. J Plant Signaling Behavior, in press.

Yang F, Mitra P, Zhang L, Prak L, Verhertbruggen Y, Kim JK, Sun L, Zheng K, Tang K, Auer M, Scheller HV and Loqué D. (2012) Engineering secondary cell wall deposition in plants. Plant Biotechnol J 3: 325-335.

Chen X, Vega-Sánchez ME, Bartley L, Verhertbruggen Y, Chiniquy D, Canlas P, Fagerström A, Prak L, Christensen U, Oikawa A, Chen M, Zuo S, Auer M, Willats W G.T., Harholt J, Scheller HV, and Ronald PC. Inactivation of OsIRX10 leads to decreased xylan content in rice stem cell walls and improved biomass saccharification. Mol Plant, (2012) doi: 10.1093/mp/sss135.  First published online: November 23, 2012

Liwanag AJM, Ebert B, Verhertbruggen Y, Rennie EA, Rautengarten C, Oikawa A, Andersen MCF, Clausen MH, Scheller HV. (2012) Pectin Biosynthesis: GALS1 in Arabidopsis thaliana is a β-1,4-galactan β-1,4-galactosyltransferase. Plant Cell 24: 12 5024-5036.

Petersen PD, Lau J, Ebert B, Yang F, Verhertbruggen Y, Kim JS, Varanasi P, Suttangkakul A, Auer M, Loqué D, Scheller HV. (2012) Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants. Biotechnol Biofuels 5: 84.

Yang F, Mitra P, Zhang L, Prak L, Verhertbruggen Y, Kim JK, Sun L, Zheng K, Tang K, Auer M, Scheller HV and Loqué D. Engineering secondary cell wall deposition in plants. Plant Biotechnol J, Published Online: 12 NOV 2012, DOI: 10.1111/pbi.12016

Rennie EA, Hansen SF, Baidoo EEK, Hadi M, Keasling JD, Scheller HV (2012) Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases. Plant Physiol 159, 1408-1417.

Harholt J, Sørensen I, Roberts A, Willats W, Scheller HV, Petersen BL, Banks JA, Ulvskov P. (2012). The glycosyltransferase repertoire of the spikemoss Selaginella moellendorffii and a comparative study of the cell wall structure. Plos One 7(5): e35846.

Hansen SF, Harholt J, Oikawa A, Scheller HV. (2012) Plant glycosyltransferases beyond CAZy: A perspective on DUF families. Frontiers Plant Physiol, 3:59

Parsons HT, Christiansen K, Knierim B, Carroll A, Ito J, Batth TS, xSmith-Moritz AM, Morrison S, McInerney P, Hadi MZ, Auer M, Mukhopadhyay A, Christiansen K, Scheller HV, Loque D, Heazlewood JL (2012). Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis. Plant Physiol 159: 12-26

Vega-Sánchez ME, Verhertbruggen Y, Christensen U, Chen X, Sharma V, Varanasi P, Jobling SA, Talbot M, White, RG, Joo M, Singh S, Auer M, Scheller HV, Ronald PC (2012). Loss of Cellulose Synthase-Like F6 function affects mixed-linkage glucan deposition, cell wall mechanical properties and defense responses in vegetative tissues of rice.  Plant Physiol 159: 56-69

Harholt J, Jensen JK, Verhertbruggen Y, Søgaard C, Bernard S, Nafisi M, Poulsen CP, Geshi N, Sakuragi Y, Driouich A, Knox JP, Scheller HV (2012). ARAD proteins associated with pectic Arabinan biosynthesis form complexes when transiently overexpressed in planta. Planta. 2012 Jul;236(1):115-28. Epub 2012 Jan 21.

Orfila C, Degan FD, Jørgensen B, Scheller HV, Ray PM, Ulvskov P (2012). Expression of mung bean pectin acetyl esterase in potato tubers: effect on acetylation of cell wall polymers and tuber mechanical properties.  Planta. 2012 Jul;236(1):185-96.

Søgaard C, Stenbæk A, Bernard S, Hadi M, Driouich A, Scheller HV, Sakuragi Y (2012) GO-PROMTO illuminates protein membrane topologies of glycan biosynthetic enzymes in the Golgi apparatus of living tissues. PLoS ONE 7: e31324.

Rautengarten C, Ebert B, Ouellet M, Nafisi M, Baidoo EEK, Benke P, Stranne M, Mukhopadhyay A, Keasling JD, Sakuragi Y, Scheller HV (2012). Arabidopsis Deficient in Cutin Ferulate encodes a transferase required for ferulyolation of w-hydroxyfatty acids in cutin polyester. Plant Physiol 158: 654-665.

Chiniquy D, Sharma V, Schultink A, Baidoo EE, Rautengarten C, Cheng K, Carroll A, Ulvskov P, Harholt J, Keasling JD, Pauly M, Scheller HV, and Ronald PC. (2012) XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan. Proc Natl Acad Sci USA 109: 17117-17122.

Oikawa A, Lund CH, Sakuragi Y, Scheller HV. Golgi-localized enzyme complexes for plant cell wall biosynthesis. Published by Elsevier Ltd. http://dx.doi.org/10.1016/j.tplants.2012.07.002 Trends in Plant Science xx (2012) 1–10

Christensen U, Scheller HV (2012) Regulation of (1,3;1,4)-b-d-glucan synthesis in developing endosperm of barley lys mutants. J Cereal Sci 55: 69–76.

2011

Atmodjo MA, Sakuragi Y, Zhu X, Burrell A, Mohanty SS, Atwood JA, Orlando R, Scheller HV, Mohnen D (2011) Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall  pectin biosynthetic homogalacturonan:galacturonosyl-transferase complex. Proc Natl Acad Sci USA 108:20225-20230.

Verhertbruggen Y, Yin L, Oikawa A and Scheller HV (2011) Mannan synthase activity in the CSLD family. J Plant Signaling Behavior 6: 1620-1623.

Banks et al. The compact Selaginella genome identifies changes in gene content associated with the evolution of vascular plants. Science. 2011 May 20; 332(6032): 960–963.  Published online 2011 May 5. doi:  10.1126/science.1203810

Rautengarten C, Ebert B, Herter T, Petzold CJ, Ishii T, Mukhopadhyay A, Usadel B, Scheller HV (2011) The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose is Indispensable for Plant Development in Arabidopsis thaliana. Plant Cell, 23: 1373–1390.

Yin L, Verhertbruggen Y, Oikawa A, Manisseri C, Knierim B, Prak L, Jensen JK, Knox JP, Willats WGT, Scheller HV (2011) The cooperative activities of CSLD2, CSLD3 and CSLD5 are required for normal Arabidopsis development. Mol Plant, 4: 1024-1037.

Sakuragi Y, Nørholm MHH, Scheller HV (2011) Visual Mapping of Cell Wall Biosynthesis. Book Chapter In: The Plant Cell Wall: Methods and Protocols, Ed. by Z. Popper. Springer Science, Berlin, Germany, pp. 153-167.

Manabe Y, Nafisi M, Verhertbruggen Y, Orfila C, Gille S, Rautengarten C, Cherk C, Marcus SE, Somerville S, Pauly M, Knox JP, Sakuragi Y, Scheller HV (2011). Loss-of-function mutation of REDUCED WALL ACETYLATION 2 in Arabidopsis leads to reduced cell wall acetylation and increased resistance to Botrytis cinerea. Plant Physiol, 155:1068-1078.

Geshi N, H.J., Sakuragi Y, Jensen JK, Scheller HV. (2011). CAZy Glycosyltransferase family 47. In Plant polysaccharides - biosynthesis and bioengineering (P. Ulvskov and N. Carpita, eds.), Wiley-Blackwell, Oxford, UK, pp. 265-284.

2010

Oikawa A, Joshi HJ, Rennie EA, Ebert B, Manisseri, C., Heazlewood JL, Scheller HV (2010) An integrative approach to the identification of arabidopsis and rice genes involved in xylan and secondary wall development. Plos One 5: e15481.

Harholt J, Suttangkakul A, Scheller HV (2010) Biosynthesis of pectin. Plant Physiol 153: 384-395.

Arora R, Manisseri C, Li C, Ong M, Scheller HV, Vogel K, Simmons BA, Singh S. (2010) Monitoring and Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment of Switchgrass (Panicum virgatum). Bioenergy Res 3: 134-145.

Scheller HV, Singh S, Blanch H, Keasling JD (2010) The Joint BioEnergy Institute (JBEI): Developing New Biofuels by Overcoming Biomass Recalcitrance. Bioenergy Res 3: 105-107.

Rautengarten C, Baidoo E, Keasling JD, Scheller HV (2010) A simple method for enzymatic synthesis of unlabeled and radiolabeled hydroxycinnamate-CoA. Bioenergy Res 3: 115-122.

Liepman AH, Wightman R, Geshi N, Turner SR, Scheller HV (2010) Arabidopsis – a Powerful Model System for Plant Cell Wall Research. Plant J 61: 1107-1121.

Scheller HV, Ulvskov P (2010) Hemicelluloses. Ann Rev Plant Biol 61: 263-289.

Vogel JP, Garvin DF, Mockler TC, Schmutz J, Rokhsar D, Bevan MW, Barry K, Lucas S, Harmon-Smith M, Lail K, Tice H, Grimwood J, McKenzie N, Huo N, Gu YQ, Lazo GR, Anderson OD, You F, Luo M-C, Dvorak J, Wright J, Febrer M, Idziak D, Hasterok R, Lindquist E, Wang M, Fox SE, Priest HD, Filichkin SA, Givan SA, Bryant DW, Chang JH, Mockler TC, Wu H, Wu W, Hsia A-P, Schnable PS, Kalyanaraman A, Barbazuk B, Michael TP, Hazen SP, Bragg JN, Laudencia-Chingcuanco D, Weng Y, Haberer G, Spannagl M, Mayer K, Rattei T, Mitros T, Lee S-J, Rose JKC, Mueller LA, York TL, Wicker T, Buchmann JP, Tanskanen J, Schulman AH, Gundlach H, de Oliveira AC, Maia LdC, Belknap W, Jiang N, Lai J, Zhu L, Ma J, Salse J, Murat F, Abrouk M, Bruggmann R, Fahlgren N, Sullivan CM, Carrington JC, Chapman E, May GD, Zhai J, Ganssmann M, Gurazada GR, German M, Meyers BC, Green PJ, Tyler L, Thomson J, Chen S, Scheller HV, Harholt J, Ulvskov P, Kimbrel JA, Chang JH, Bartley LE, Cao P, Jung K-H, Sharma MK, Vega-Sanchez M, Ronald P, Dardick CD, DeBodt S, Verelst W, Inzé D, Heese M, Schnittger A, Yang X, Kalluri U, Tuskan GA, Hua Z, Vierstra RD, Cui Y, Ouyang S, Sun Q, Liu Z, Yilmaz A, Grotewold E, Sibout R, Hematy K, Mouille G, Höfte H, Michael T, Pelloux J, O’Connor D, Schnable J, Rowe S, Harmon F, Cass CL, Sedbrook JC, Byrne M, Walsh S, Higgins J, Li P, Brutnell T, Turgay U, Budak H, Belcram H, Charles M, Chalhoub B, Baxter I. (2010) Genome sequencing analysis of the model grass Brachypodium distachyon. Nature 463: 763-768.

Harholt J, Bach IC, Lind-Bouquin S, Nunan KJ, Madrid SM, Brinch-Pedersen H, Holm PB, Scheller HV (2010). Generation of transgenic wheat (Triticum aestivum L.) accumulating heterologous endo-xylanase and ferulic acid esterase in the endosperm. Plant Biotechnol J 8: 351-362.

Christensen U, Alonso-Simon A, Scheller HV, Willats WGT, Harholt J (2010) Characterization of the primary cell walls of seedlings of Brachypodium distachyon – A potential model plant for temperate grasses. Phytochemistry 71: 62-69.

Geshi N, Petersen BL, Scheller HV (2010) Towards tailored synthesis of functional polysaccharides in plants. In ‘Foods for Health in the 21st Century: A Road Map for the Future’. (Eds. ME Gershwin & MRC Greenwood) Ann NY Acad Sci 1190: 50-57.

Li C, Knierim B, Manisseri C, Arora R, Scheller HV, Auer M, Vogel K, Simmons BA, Singh S (2010) Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification. Bioresource Technol 101: 4900-4906

2009

Landau AM, Lokstein H, Scheller HV, Lainez V, Maldonado S, Prina AR (2009) A cytoplasmically inherited barley mutant is defective in Photosystem I assembly due to a temperature sensitive defect in ycf3 splicing. Plant Physiol 151: 1802–1811.

Petersen BL, Egelund J, Damager I, Faber K, Jensen JK, Yang Z, Bennett EP, Scheller HV, Ulvskov P (2009) Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases. Glycoconjugate J 26: 1235-1246.

Takahashi J, Rudsander UJ, Hedenström M, Banasiak A, Harholt J, Amelot N, Immerzeel P, Ryden P, Endo S, Ibatullin FM, Brumer H, del Campillo E, Master ER, Scheller HV, Sundberg B, Teeri TT, Mellerowicz EJ (2009) KORRIGAN1 and its aspen homolog PttCel9A1 decrease cellulose crystallinity in Arabidopsis stems. Plant Cell Physiol 50:1099-1115

Meng M, Geisler M, Johansson H, Harholt J, Scheller HV, Mellerowicz EJ, Kleczkowski LA (2009) UDP-glucose pyrophosphorylase is not rate-limiting, but is essential in Arabidopsis. Plant Cell Physiol 50: 998-1011.

Fristedt R, Carlberg I, Zygadlo A, Piippo M, Nurmi M, Aro E-M, Scheller HV, Vener AV (2009). Intrinsically Unstructured Phosphoprotein TSP9 Regulates Light Harvesting in Arabidopsis thaliana. Biochemistry 48: 499-509.

2008

Bernal AJ, Yoo C-M, Mutwil M, Jensen JK, Hou G, Blaukopf C, Sørensen I, Blancaflor EB, Scheller HV, Willats WGT (2008) Functional Analysis of the Cellulose Synthase Like Genes ATCSLD1, ATCSLD2 and ATCSLD4 In Tip Growing Arabidopsis Cells. Plant Physiol 148: 1238-1253

Sirpiö S, Khrouchtchova A, Allahverdiyeva Y, Hansson M, Fristedt R, Vener A, Scheller HV, Jensen PE, Haldrup A, Aro E-M (2008) AtCYP38 ensures early biogenesis, correct assembly and sustenance of photosystem II. Plant J 55: 639–651.

Jensen JK, Sørensen SO, Harholt J, Sakuragi Y, Møller I, Zandleven J, Bernal AJ, Jensen NB, Sørensen C, Pauly M, Beldman G, Willats WGT, Scheller HV (2008) Identification of a xylogalacturonan xylosyltransferase involved in pectin biosynthesis in Arabidopsis. Plant Cell 20: 1289-1302

Vainonen J, Sakuragi Y, Stael S, Tikkanen M, Allahverdiyeva Y, Paakkarinen V, Aro E, Suorsa M, Scheller HV, Vener A, Aro E-M (2008) Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana. FEBS J, 275: 1767–1777.

2007

Bernal AJ, Jensen JK, Harholt J, Sørensen SO, Møller I, Blaukopf C, Johansen B, de Lotto R, Pauly M, Scheller HV, Willats WGT (2007) Disruption of ATCSLD5 results in reduced growth, reduced xylan and homogalacturonan synthase activity and altered xylan occurrence in Arabidopsis. Plant J 52: 791-802.

Jensen PE, Bassi R, Boekema EJ, Dekker JP, Jansson S, Leister D, Robinson C, Scheller HV (2007) Structure, function and regulation of green plant photosystem I. Biochim Biophys Acta 1767: 335-352.

Zandleven J, Sørensen SO, Harholt J, Beldman G, Schols HA, Scheller HV, Voragen AGJ (2007) Xylogalacturonan exists in cell walls from various tissues of Arabidopsis thaliana. Phytochemistry 68: 1219-1226.

Hansson A, Amann K, Zygadlo A, Meurer J, Scheller HV, Jensen PE (2007) Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum. PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J 274: 1734–1746.

Scheller HV, Jensen JK, Sørensen SO, Harholt J, Geshi N (2007) Biosynthesis of pectin. Physiol Plant 129: 283-295

2006

Haldrup A, Jensen PE, Scheller HV (2006) The Low Molecular Mass Subunits in Higher Plant Photosystem I. In: Photosystem I: the Plastocyanin:Ferredoxin Oxidoreductase in Photosynthesis (JH Golbeck, ed.), in press, Kluwer Academic Publishers, The Netherlands

Zygadlo A, Robinson C, Scheller HV, Mant A, Jensen PE (2006) The properties of the positively charged loop region in PSI-G are essential for its “spontaneous” insertion into thylakoids and rapid assembly into the photosystem I complex. J Biol Chem 281: 10548-10554.

Harholt J, Jensen JK, Sørensen SO, Orfila C, Pauly M, Scheller HV (2006) ARABINAN DEFICIENT 1 is a Putative Arabinosyltransferase Involved in Biosynthesis of Pectic Arabinan in Arabidopsis. Plant Physiol 140: 49-58.

2005

Khrouchtchova A, Hansson M, Paakkarinen V, Vainonen JP Zhang S, Jensen PE, Scheller HV, Vener AV, Aro E-M, Haldrup A (2005) A previously found thylakoid membrane protein of 14 kDa (TMP14) is a novel subunit of plant photosystem I and is designated PSI-P. FEBS Lett 579: 4808-4812.

Kouril R, Zygadlo A, Arteni AA, de Wit CD, Dekker JP, Jensen PE, Scheller HV, Boekema EJ. Structural characterization of a complex of photosystem I and light-harvesting complex II of Arabidopsis thaliana. Biochemistry 33: 10935-10940.

Rosgaard L, Zygadlo A, Scheller HV, Mant A, Jensen PE. Insertion of the plant photosystem I subunit G into the thylakoid membrane: in vitro and in vivo studies of wild-type and tagged versions of the protein. FEBS J 272, 4002-4010.

Singh SK, Eland C, Harholt J, Scheller HV, Marchant A. Cell adhesion in Arabidopsis thaliana is mediated by ECTOPICALLY PARTING CELLS 1 - a glycosyltransferase (GT64) related to the animal exostosins (2005). Plant J 43: 384-397.

Orfila C, Sørensen SO, Harholt J, Geshi N, Crombie H, Truong H-N, Reid JSG, Knox JP, Scheller HV (2005). QUASIMODO1 is expressed in vascular tissue of Arabidopsis thaliana inflorescence stems, and affects homogalacturonan and xylan biosynthesis. Planta 222: 613 – 622..

Zygadlo A, Jensen PE, Leister D, Scheller HV (2005) Photosystem I lacking the PSI-G subunit has a higher affinity for plastocyanin and is sensitive to photodamage. Biochim Biophys Acta 1708, 154-163.

Scheller HV, Haldrup A (2005) Photoinhibition of photosystem I. Planta 221, 5-8.

Klimmek F, Ganeteg U, Ihalainen JA, van Roon H, Jensen PE, Scheller HV, Dekker JP, Jansson S (2005) Structure of higher plant Light Harvesting Complex I: In vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44, 3065-3073.

Scheller HV, Lunde C, Haldrup A, Jensen PE (2005) Functional characterisation of the photosynthetic apparatus in Arabidopsis thaliana. In: Plant Functional Genomics (D. Leister, ed.), Haworth's Food Products Press, Binghamton, New York, pp. 393-429.

Courses

199 - Supervised Independent Study

200E - Plant Biochemistry